Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy

Fenton-like reaction-associated chemodynamic therapy (CDT) and hyperthermia-inducing photothermal therapy (PTT)-combined crosslinked hydrogel systems were developed for loco-regional cancer therapy. Cupric sulfate (Cu) has been employed to crosslink the catechol-functionalized hyaluronic acid (HC) polymer-based gel via metal-catechol coordination and covalent bonding of the catechol group (by pH adjustment). Cu can also be used as a hydroxyl radical-generating agent with endogenous H2O2 in cancer cells mediated by Fenton-like reaction and it can reduce intracellular glutathione (GSH) levels leading to the inhibition of reactive oxygen species (ROS) scavenging.
These two strategies can amplify the ROS-initiated CDT efficiency for combating cancer. The Cu-incorporated crosslinked hydrogel structure with pH modulation was appropriate for injectable gel formation via a single syringe. The incorporation of indocyanine green (ICG) into the hydrogel network and near-infrared (NIR) laser irradiation provided a temperature elevation sufficient for induction of hyperthermia in cancer therapy. It is expected that the designed HC/Cu/ICG hydrogel can be used safely and efficiently for local CDT and PTT of breast cancer.

The Role of Plasmapheresis in Treating Lethal Cupric Sulfate Poisoning.

The mortality rate of cupric sulfate is relatively high in contrast to that of other heavy metals. Cases of orally ingested cupric sulfate poisoning are very rare, with a reported half lethal dose of 10 g. Cupric sulfate poisoning leads to gastrointestinal corrosion, intravascular hemolysis, hemolytic anemia, methemoglobinemia and acute renal and hepatic impairment. Without proper and prompt treatment, multiple organ failure and death occur. Here, we present the first report that removal of the excessive intravascular copper ions by plasmapheresis was accompanied by complete recovery.

Preparation of cupric sulfate-based self-emulsifiable nanocomposites and their application to the photothermal therapy of colon adenocarcinoma.

Nanocomposites (NCs) of cupric sulfate monohydrate (CuSO4) were fabricated by hot-melt extrusion (HME) system equipped with twin screws. Micron-sized bulk powder of CuSO4 was dispersed in the mixture of surfactants (Span 80 and Tween 80) and hydrophilic polymer (polyethylene glycol (PEG) 6000) by HME process. Reduction of surface tension by surfactants and homogeneous dispersion in hydrophilic polymer along with HME technique were introduced to prepare CuSO4 NCs.
Dispersion of CuSO4 NCs exhibited approximately 204 nm hydrodynamic size, unimodal size distribution, and positive zeta potential values. Encapsulation of CuSO4 in CuSO4 NCs and the physicochemical interactions between CuSO4 and pharmaceutical excipients were investigated by solid-state studies. Of note, CuSO4 NCs group exhibited higher antiproliferation efficacies, compared with bulk CuSO4, in Caco-2 (human adenocarcinoma) cells at 75 and 100 μg/mL CuSO4 concentrations (p < 0.05). Also, near-infrared laser irradiation to CuSO4 NCs group elevated the antiproliferation efficacies, compared with non-irradiation group, in Caco-2 cells. After intravenous injection in mice, CuSO4 NCs did not show severe in vivo toxicities. Developed CuSO4 NCs can be one of promising candidates of photothermal therapeutic agents for colon cancers.

Ultralow Sample Volume Cupric Sulfate Oxidation Method for the Analysis of Dissolved Lignin.

A novel cupric sulfate (CuSO4) oxidation method was developed to enable the analysis of dissolved lignin phenols in small volumes of open ocean seawater (<200 mL for deep ocean) or river water (<17 mL) samples. Dissolved lignin phenols were isolated by automated reversed-phase (octadecyl) extraction from seawater before oxidation, whereas for freshwater samples, oxidation was performed without prior extraction. Optimized reaction conditions using alkaline CuSO4 at 150 °C effectively limited losses of lignin phenols and suppressed side reactions at 5-100 μg of sample organic carbon.
The method yielded up to ∼33% higher lignin phenol concentrations and 24-36% lower acid/aldehyde ratios of lignin phenols than existing CuO oxidation methods. The microscale design (200 μL reaction volume) resulted in extremely low blanks allowing accurate and precise quantification of lignin phenols. A comparison of silica-based octadecyl-bonded sorbents (C18) with copolymer-type sorbents (PPL) indicated that sorbent type affected concentrations and diagnostic ratios of dissolved lignin phenols. For robust intercomparability of measured lignin phenol concentrations and in consideration of method detection limits for quantification, a constant sample organic carbon content of ∼30 μg C and the addition of 150 μg ascorbic acid-C are recommended. Small sample volumes avoid time-consuming extraction steps in the field, and the simplified oxidation and sample processing procedures make the new method ideal for high-throughput analysis of dissolved lignin phenols in marine and freshwater environments.

Dioxins contamination in the feed additive (feed grade cupric sulfate) tied to chlorine industry.

The sources of polychlorinated dibenzo-p-dioxins/dibenzofurans (PCDD/Fs) found in animal feed additive (feed grade cupric sulfate, CuSO4) were investigated and traced back to the formation of chlorinated organic compounds in the chlor-alkali industry. PCDD/Fs could be transported through the supply chain: hydrochloric acid (HCl) by-produced during formation of chlorinated organic compounds in chlor-alkali industry → spent acid etching solution (acid-SES) generated in printed circuit board production → industrial cupric salt → CuSO4 in animal feed, and finally enter the food chain.
The concentration ranges in HCl and acid-SES were similar, of which the level in acid-SES was also consistent with that in various cupric salt products including CuSO4 based on Cu element content. PCDD/Fs also showed very similar congener profiles in all the sample types. This indicates a probable direct transport pathway of PCDD/Fs into the food chain, which may eventually be exposed to humans through consumption. To date this is the first study in China that systematically reports on the PCDD/Fs transport from industrial pollution sources to industrial processes and finally enters the human food chain.

Synergistic antifungal activity of sodium hypochlorite, hydrogen peroxide, and cupric sulfate against Penicillium digitatum.

Oxidizing compounds such as sodium hypochlorite (NaCIO) and hydrogen peroxide (H2O2) are widely used in food sanitization because of their antimicrobial effects. We applied these compounds and metals to analyze their antifungal activity against Penicillium digitatum, the causal agent of citrus green mold. The MICs were 300 ppm for NaClO and 300 mM for H2O2 when these compounds were individually applied for 2 min to conidia suspensions. To minimize the concentration of these compounds, we developed and standardized a sequential treatment for conidia that resulted in loss of viability on growth plates and loss of infectivity on lemons.

isa cupric

from Consort
ISA25 | ea: 91.00 EUR

Cupric chloride

from Abbexa
abx186417-5g | 5 g: 189.00 EUR

calibration solution cupric

from Consort
ISC25 | ea: 91.00 EUR

Cupric Bromide,95%

from Abbexa
abx188661-25g | 25 g: 286.00 EUR

Quinine Sulfate

from TOKU-E
Q012-10G | 10g: 53.00 EUR

Quinine Sulfate

from TOKU-E
Q012-25G | 25g: 64.00 EUR

Quinine Sulfate

from TOKU-E
Q012-50G | 50g: 153.00 EUR

Sisomicin sulfate

from TOKU-E
S004-1G | 1 g: 219.00 EUR

Sisomicin sulfate

from TOKU-E
S004-500MG | 500 mg: 138.00 EUR

Streptomycin Sulfate

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S008-100G | 100 g: 77.00 EUR

Streptomycin Sulfate

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S008-1kG | 1 kg: 402.00 EUR

Streptomycin Sulfate

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S008-25G | 25 g: 48.00 EUR

Spectinomycin sulfate

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S016-1G | 1 g: 49.00 EUR

Spectinomycin sulfate

from TOKU-E
S016-5G | 5 g: 105.00 EUR

Netilmicin sulfate

from TOKU-E
N004-5MG | 5 mg: 509.00 EUR

Micronomicin sulfate

from TOKU-E
M018-10G | 10 g: 100.00 EUR

Paromomycin sulfate

from TOKU-E
P002-25G | 25 g: 499.00 EUR

Paromomycin sulfate

from TOKU-E
P002-5G | 5 g: 171.00 EUR

Vinblastine (sulfate)

from MedChemExpress
HY-13780 | 10mg: 139.00 EUR
The in vitro treatment consists of preincubation with 10 ppm of NaClO followed by incubation with 100 mM H2O2 and 6 mM CuSO4 (cupric sulfate). The combination of NaClO and H2O2 in the presence of CuSO4 produces a synergistic effect (fractional inhibitory concentration index of 0.36). The sequential treatment applied in situ on lemon peel 24 h after the fruit was inoculated with conidia produced a significant delay in the fungal infection. The in vitro treatment was effective on both imazalil-sensitive and imazalil-resistant strains of P. digitatum and Geotrichum candidum, the causal agent of citrus sour rot. However, this treatment inhibited 90% of mycelial growth for Penicillium italicum (citrus blue mold). These results indicate that sequential treatment may be useful for postharvest control of citrus fruit diseases.

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