Category: Optimization of 5′ Untranslated Region of Modified mRNA for Use in Cardiac or Hepatic Ischemic Injury.

Circadian Control of REDOX Reactions in the Macrophage Inflammatory Response

On: By: Brent
Significance: Macrophages are immune sentinels located throughout the body that function in both the amplification and resolution of the inflammatory response. The circadian clock has emerged as a central regulator of macrophage inflammation. Reduction-oxidation (REDOX) reactions are central to both circadian clock and macrophage function. Recent Advances: Circadian regulation of metabolism controls the macrophage inflammatory response, whereby disruption of the clock causes dysfunctional inflammation. Altering metabolism and reactive oxygen/nitrogen species (RONS) production rescues the inflammatory phenotype of clock-disrupted macrophages.
Critical issues: The circadian clock possesses many layers of regulation. Understanding how REDOX reactions coordinate clock function is critical to uncover the full extent of circadian regulation of macrophage inflammation. We provide insights into how circadian regulation of REDOX affects macrophage pattern recognition receptor signaling, immunometabolism, phagocytosis, and inflammasome activation.
Future directions: Many diseases associated with aberrant macrophage derived inflammation exhibit time of day rhythms in disease symptoms and severity and are sensitive to circadian disruption. Macrophage function is highly dependent on REDOX reactions that signal through RONS. Future studies are needed to evaluate the extent of circadian control of macrophage inflammation, specifically in the context of REDOX signaling.

Sarcopenia Is Associated with Metabolic Syndrome in Korean Adults Aged over 50 Years: A Cross-Sectional Study

This study assessed the association between sarcopenia and metabolic syndrome in Korean adults aged over 50 years. The study obtained data from the Korea National Health and Nutrition Examination Survey (KNHANES, 2008-2011), a cross-sectional and nationally representative survey conducted by the Korean Centers for Disease Control and Prevention. Among the 8363 participants included in this study, the prevalence rate of sarcopenia according to metabolic syndrome was stratified by sex.
Crude odds ratios not adjusted for any variables were 1.827 (1.496-2.231) in males, 2.189 (1.818-2.635) in females, and 2.209 (1.766-2.331) in total participants compared with non-sarcopenia. Model 3, which was adjusted for all variables that could affect sarcopenia and metabolic syndrome, showed significant increases in the odds ratios, to 1.957 (1.587-2.413) in males, 1.779 (1.478-2.141) in females, and 1.822 (1.586-2.095) for total participants. The results suggest that the association between sarcopenia and metabolic syndrome is significant in Korean adults.

Genome-Wide Characterization of SARS-CoV-2 Cytopathogenic Proteins in the Search of Antiviral Targets

Therapeutic inhibition of critical viral functions is important for curtailing coronavirus disease 2019 (COVID-19). We sought to identify antiviral targets through the genome-wide characterization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) proteins that are crucial for viral pathogenesis and that cause harmful cytopathogenic effects.
All 29 viral proteins were tested in a fission yeast cell-based system using inducible gene expression. Twelve proteins, including eight nonstructural proteins (NSP1, NSP3, NSP4, NSP5, NSP6, NSP13, NSP14, and NSP15) and four accessory proteins (ORF3a, ORF6, ORF7a, and ORF7b), were identified that altered cellular proliferation and integrity and induced cell death. Cell death correlated with the activation of cellular oxidative stress. Of the 12 proteins, ORF3a was chosen for further study in mammalian cells because it plays an important role in viral pathogenesis and its activities are linked to lung tissue damage and a cytokine storm. In human pulmonary and kidney epithelial cells, ORF3a induced cellular oxidative stress associated with apoptosis and necrosis and caused activation of proinflammatory response with production of the cytokines tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6), and IFN-β1, possibly through the activation of nuclear factor kappa B (NF-κB).
To further characterize the mechanism, we tested a natural ORF3a Beta variant, Q57H, and a mutant with deletion of the highly conserved residue, ΔG188.
Compared with wild-type ORF3a, the ΔG188 variant yielded more robust activation of cellular oxidative stress, cell death, and innate immune response. Since cellular oxidative stress and inflammation contribute to cell death and tissue damage linked to the severity of COVID-19, our findings suggest that ORF3a is a promising, novel therapeutic target against COVID-19.
IMPORTANCE The ongoing COVID-19 pandemic caused by SARS-CoV-2 has claimed over 5.5 million lives with more than 300 million people infected worldwide. While vaccines are effective, the emergence of new viral variants could jeopardize vaccine protection. Treatment of COVID-19 by antiviral drugs provides an alternative to battle against the disease. The goal of this study was to identify viral therapeutic targets that can be used in antiviral drug discovery.
Utilizing a genome-wide functional analysis in a fission yeast cell-based system, we identified 12 viral candidates, including ORF3a, which cause cellular oxidative stress, inflammation, apoptosis, and necrosis that contribute to cytopathogenicity and COVID-19. Our findings indicate that antiviral agents targeting ORF3a could have a great impact on COVID-19.

Copolymers Containing 1-Methyl-2-phenyl-imidazole Moieties as Permanent Dipole Generating Units: Synthesis, Spectroscopic, Electrochemical, and Photovoltaic Properties

New donor-acceptor conjugated alternating or random copolymers containing 1-methyl-2-phenylbenzimidazole and benzothiadiazole (P1), diketopyrrolopyrrole (P4), or both acceptors (P2) are reported. The specific feature of these copolymers is the presence of a permanent dipole-bearing moiety (1-methyl-2-phenyl imidazole (MPI)) fused with the 1,4-phenylene ring of the polymer main chain.
For comparative reasons, polymers of the same main chain but deprived of the MPI group were prepared, namely, P5 with diketopyrrolopyrrole and P3 with both acceptors. The presence of the permanent dipole results in an increase of the optical band gap from 1.51 eV in P3 to 1.57 eV in P2 and from 1.49 eV in P5 to 1.55 eV in P4. It also has a measurable effect on the ionization potential (IP) and electrochemical band gap (EgCV), leading to their decrease from 5.00 and 1.83 eV in P3 to 4.92 and 1.79 eV in P2 as well as from 5.09 and 1.87 eV in P5 to 4.94 and 1.81 eV in P4. 
Moreover, the presence of permanent dipole lowers the exciton binding energy (Eb) from 0.32 eV in P3 to 0.22 eV in P2 and from 0.38 eV in P5 to 0.26 eV in P4. These dipole-induced changes in the polymer properties should be beneficial for photovoltaic applications. Bulk heterojunction solar cells fabricated from these polymers (with PC71BM acceptor) show low series resistance (rs), indicating good electrical transport properties. The measured power conversion efficiency (PCE) of 0.54% is limited by the unfavorable morphology of the active layer.

Preparation of Naringenin Nanosuspension and Its Antitussive and Expectorant Effects

Naringenin (NRG) is a natural flavonoid compound abundantly present in citrus fruits and has the potential to treat respiratory disorders. However, the clinical therapeutic effect of NRG is limited by its low bioavailability due to poor solubility.
To enhance the solubility, naringenin nanosuspensions (NRG-NSps) were prepared by applying tocopherol polyethylene glycol succinate (TPGS) as the nanocarrier via the media-milling method.

Formulated DMEM

 from Addexbio
C0003-01 | RT 500 mL Bottle: 98.00 EUR

Optimized DMEM

 from Addexbio
C0003-02 | RT 500 mL Bottle: 100.00 EUR

Specialized DMEM

 from Addexbio
C0003-03 | RT 500 mL Bottle: 104.00 EUR

DMEM, High Glucose

 from GenDepot
CM001-050 | 500ml: 72.00 EUR

DMEM, High Glucose

 from GenDepot
CM001-300 | 6x500ml: 158.00 EUR

DMEM, High Glucose

 from GenDepot
CM001-310 | 10x500ml: 201.00 EUR

DMEM, High Glucose

 from GenDepot
CM001-320 | 20x500ml: 313.00 EUR

DMEM, High Glucose

 from GenDepot
CM001-350 | 50x500ml: 571.00 EUR

DMEM, High Glucose

 from GenDepot
CM002-050 | 500ml: 72.00 EUR

DMEM, High Glucose

 from GenDepot
CM002-300 | 6x500ml: 158.00 EUR

DMEM, High Glucose

 from GenDepot
CM002-310 | 10x500ml: 201.00 EUR

DMEM, High Glucose

 from GenDepot
CM002-320 | 20x500ml: 313.00 EUR

DMEM, High Glucose

 from GenDepot
CM002-350 | 50x500ml: 571.00 EUR

DMEM, High Glucose

 from GenDepot
CM003-050 | 500ml: 74.00 EUR

DMEM, High Glucose

 from GenDepot
CM003-300 | 6x500ml: 165.00 EUR
The particle size, morphology, and drug-loading content of NRG-NSps were examined, and the stability was evaluated by detecting particle size changes in different physiological media. NRG-NSps exhibited a flaky appearance with a mean diameter of 216.9 nm, and the drug-loading content was 66.7%. NRG-NSps exhibited good storage stability and media stability. NRG-NSps presented a sustainable release profile, and the cumulative drug-release rate approached approximately 95% within 7 d. NRG-NSps improved the antitussive effect significantly compared with the original NRG, the cough frequency was decreased from 22 to 15 times, and the cough incubation period was prolonged from 85.3 to 121.6 s.
Besides, NRG-NSps also enhanced expectorant effects significantly, and phenol red secretion was increased from 1.02 to 1.45 μg/mL. These results indicate that NRG-NSps could enhance the bioavailability of NRG significantly and possess a potential clinical application.

Microbe-derived antioxidants attenuate cobalt chloride-induced mitochondrial function, autophagy and BNIP3-dependent mitophagy pathways in BRL3A cells

On: By: Brent
Environmental excessive cobalt (Co) exposure increases risks of public health. This study aimed to evaluate the potential mechanism of microbe-derived antioxidants (MA) blend fermented by probiotics in attenuating cobalt chloride (CoCl2)-induced toxicology in buffalo rat liver (BRL3A) cells. Herein, results showed that some phenolic acids increased in MA compared with the samples before fermentation through UHPLC-QTOF-MS analysis. Also, the contents of essential and non-essential amino acids, their derivatives and minerals were rich in MA. The DPPH, O2–, OH– and ABTS+ scavenging ability of MA is comparable to those of vitamin C and better than mitoquinone mesylate (mitoQ).
In vitro cell experiments showed that CoCl2 treatment increased the percentage of apoptosis cells, lactate dehydrogenase and genes involved in glycolysis, increased ATP production and decreased mitochondrial membrane potential, increased genes involved in canonical autophagy process (including initiation, autophagosomes maturation and fusion with lysosome) and BNIP3-dependent mitophagy pathways in BRL3A cells, while MA attenuated CoCl2-induced reactive oxygen species (ROS) production, apoptosis, mitochondrial protein expression and dysfunction, and BNIP3-dependent mitophagy. Collectively, these results provide insights into the role of MA in reversing CoCl2-induced toxicology in BRL3A cells, providing the promising constituents for decreasing Co-induced toxicology in functional foods.

Cardioprotective effects of alantolactone on isoproterenol-induced cardiac injury and cobalt chloride-induced cardiomyocyte injury

Objectives: Alantolactone (AL) is a compound extracted from the roots of Inula Racemosa that has shown beneficial effects in cardiovascular disease. However, the cardioprotective mechanism of AL against hypoxic/ischemic (H/I) injury is still unclear. This research aimed to determine AL’s ability to protect the heart against isoproterenol (ISO)-induced MI injury in vivo and cobalt chloride (CoCl2) induced H/I injury in vitro.
Methods: Electrocardiography (ECG), lactate dehydrogenase (LDH), creatine kinase (CK), and cardiac troponin I (cTnI) assays in addition to histological analysis of the myocardium were used to investigate the effects of AL in vivo. Influences of AL on L-type Ca2+ current (ICa-L) in isolated rat myocytes were observed by the patch-clamp technique. Furthermore, cell viability, apoptosis, oxidative stress injury, mitochondrial membrane potential, and intracellular Ca2+ concentration were examined in vitro.
Results: The results indicated that AL treatment ameliorated the morphological and ECG changes associated with MI, and decreased levels of LDH, CK, and cTnI. Furthermore, pretreatment with AL elevated antioxidant enzyme activity and suppressed ROS production. AL prevented H/I-induced apoptosis, mitochondria damage, and calcium overload while reducing ICa-L in a concentration and time dependent fashion. The 50% inhibiting concentration (IC50) and maximal inhibitory effect (Emax) of AL were 17.29 μmol/L and 57.73 ± 1.05%, respectively.
Conclusion: AL attenuated MI-related injury by reducing oxidative stress, apoptosis, calcium overload, and mitochondria damage. These cardioprotective effects may be related to the direct inhibition of ICa-L.
Keywords: L-type calcium channel currents; alantolactone; calcium influx; cardio-protection; oxidative stress.

Tongxin formula protects H9c2 cardiomyocytes from cobalt chloride-induced hypoxic injury via inhibition of apoptosis

In this study, the effect of the Tongxin formula (TXF) on the apoptosis of H9c2 cardiomyocytes induced by cobalt chloride (CoCl2) was investigated, and the potential mechanism was explored. A hypoxic injury model of H9c2 cardiomyocytes was established using CoCl2. The cell viability was measured using a Cell Counting Kit-8 assay. The lactate dehydrogenase (LDH) release and caspase-3 activity were measured using spectrophotometry. The apoptosis was measured via Annexin V-FITC/PI staining and flow cytometry. The changes in the mitochondrial membrane potential were examined using immunofluorescence microscopy following the loading of JC-1 probes.
The expressions of apoptosis-related proteins and key proteins in the phosphatidylinositol 3-kinase/protein kinase B (PI3K/Akt) pathway were examined via immunoblotting. The different TXF concentrations studied significantly improved the percentage of viability of cardiomyocytes with hypoxic injury, and the LDH release, apoptotic rate, caspase-3 activity, and levels of cleaved caspase-3 protein were reduced in the injured cells. Additionally, the TXF group had increased mitochondrial membrane potential, upregulated expression of Bcl-2 and p-Akt proteins, and significantly reduced expression of cleaved caspase-3 protein in the cells with hypoxic injury.
Moreover, in the TXF group, the treatment significantly reduced the BAX protein expression, but the difference was not statistically significant compared with the CoCl2 group. In this study, TXF regulated the expression of apoptosis-related proteins, inhibited apoptosis, increased the mitochondrial membrane potential, and alleviated damage to the mitochondrial membrane, thereby protecting the cardiomyocytes from hypoxic injury. The underlying mechanism could be related to activation of the PI3K/Akt signaling pathway and upregulation of the Bcl-2 protein.

The biological effect of cobalt chloride mimetic-hypoxia on nucleus pulposus cells and the comparability with physical hypoxia in vitro

Objective: Nucleus pulposus cells (NPCs) are cells e induce mimetic-hypoxia for NPCs and the comparison withxtracted from the intervertebral disc and are important for research into intervertebral disc degeneration (IVDD). NPCs live in an avascular and relatively hypoxic environment. Cobalt chloride (CoCl2) has been used in many cell studies to mimic hypoxia. The objective of this study was to explore the possibility of using CoCl2 to hypoxia (1% O2) in vitro.
 Materials and methods: Rat nucleus pulposus cells of Passage 3-5 were used in this research. Cell viability, rate of cell apoptosis, ROS (reactive oxygen species) generation, cell migration, extracellular pH and extracellular matrix metabolism were determined to compare the influence of hypoxia (1% O2) and CoCl2 on NPCs.
Results: We found that the effects of CoCl2 on NPCs was dose-dependent. At the proper concentration, CoCl2 could be used to elicit chemical hypoxia for nucleus pulposus cells in vitro and many biological effects, analogous to physical hypoxia (1% O2), could be achieved such as enhanced cell viability, decreased apoptosis and activated extracellular matrix metabolism. On the other hand, CoCl2 mimetic-hypoxia did not affect NPCs glycolysis and migration compared to physical hypoxia. In addition, high concentration of CoCl2 (>200 μM) is harmful to NPCs with high rates of apoptosis and ECM (extracellular matrix) degradation.
 Conclusions: It is feasible and convenient to use CoCl2 to induce chemical mimetic hypoxia for culturing NPCs on the premise of appropriate concentration. But in aspects of cell migration and glycolysis, CoCl2 could not achieve similar results with physical hypoxia. This study may provide a convenient method and enlightenment to induce mimetic-hypoxia for researchers studying NPCs and IVVD.
Keywords: Apoptosis; Cobalt chloride; Extracellular matrix synthesis; Hypoxia; Migration; Nucleus pulposus cells.

Synthesis and structure determination of racemic (Δ/Λ)-tris-(ethyl-enedi-amine)-cobalt(III) trichloride hemi(hexa-aqua-sodium chloride)

The synthesis and crystal structure of the title racemic compound, [Co(C2H8N2)3]Cl3.{[Na(H2O)6]Cl}0.5, are reported. The trivalent cobalt atom, which resides on a crystallographic threefold axis, is chelated by a single ethyl-ene di-amine (en) ligand and yields the tris-chelate [Co(en)3]3+ cation with distorted octa-hedral geometry after the application of crystal symmetry.

Cobalt(II) chloride hexahydrate

 from Glentham Life Sciences
GK0520-100G | 100 g: 51.00 EUR

Cobalt(II) chloride hexahydrate

 from Glentham Life Sciences
GK0520-1KG | 1 kg: 132.00 EUR

Cobalt(II) chloride hexahydrate

 from Glentham Life Sciences
GK0520-250G | 250 g: 66.00 EUR

Cobalt(II) chloride hexahydrate

 from Glentham Life Sciences
GK0520-25G | 25 g: 42.00 EUR

Cobalt(II) chloride hexahydrate

 from Glentham Life Sciences
GK0520-500G | 500 g: 90.00 EUR

Cobalt(II) chloride, anhydrous

 from Glentham Life Sciences
GK0658-100G | 100 g: 74.00 EUR

Cobalt(II) chloride, anhydrous

 from Glentham Life Sciences
GK0658-250G | 250 g: 110.00 EUR

Cobalt(II) chloride, anhydrous

 from Glentham Life Sciences
GK0658-25G | 25 g: 48.00 EUR

Cobalt(II) chloride, anhydrous

 from Glentham Life Sciences
GK0658-500G | 500 g: 174.00 EUR

Cobalt phthalocyanine

 from MedChemExpress
HY-18761 | 1g: 119.00 EUR

Cobalt(Ii) Oxide

 from Abbexa
abx188657-25g | 25 g: 314.00 EUR

Cobalt(Iii) Fluoride

 from Abbexa
abx188658-25g | 25 g: 370.00 EUR

High Density Cobalt

 from ABTBeads
6BCL-QHCo-100 | 100 ml: 372.00 EUR

High Density Cobalt

 from ABTBeads
6BCL-QHCo-25 | 25 ml: 162.00 EUR

High Density Cobalt

 from ABTBeads
6BCL-QHCo-500 | 500 ml: 1304.00 EUR

Cobalt Rapid Run

 from ABTBeads
6CoRR-100 | 100 ml: 431.00 EUR

Cobalt Rapid Run

 from ABTBeads
6CoRR-25 | 25 ml: 166.00 EUR

Cobalt Rapid Run

 from ABTBeads
6CoRR-500 | 500 ml: 1474.00 EUR

Cobalt Assay Kit

 from Fitzgerald
55R-1435 | 100 assays: 621.00 EUR

Cobalt(II) acetate tetrahydrate

 from Glentham Life Sciences
GK1133-100G | 100 g: 48.00 EUR
The sodium cation (site symmetry ), has a single water mol-ecule bound to it in the asymmetric unit and yields a distorted, octa-hedrally coordinated hydrated [Na(H2O)6]+ cation after the application of symmetry. One of the chloride ions lies on a general position and the other has site symmetry. An extensive array of C-H⋯O, N-H⋯Cl and O-H⋯Cl hydrogen bonds exists between the ethyl-ene di-amine ligands, the water mol-ecules of hydration, and the anions present, thereby furnishing solid-state stability.
Keywords: crystal structure; enanti­omer; ethyl­ene di­amine; racemate.

Optimization of 5′ Untranslated Region of Modified mRNA for Use in Cardiac or Hepatic Ischemic Injury.

Optimization of 5' Untranslated Region of Modified mRNA for Use in Cardiac or Hepatic Ischemic Injury.
On: By: Brent

Modified mRNA (modRNA) is a gene-delivery platform for transiently introducing a single gene or a number of genes of curiosity to totally different cell varieties and tissues. modRNA is taken into account to be a secure vector for gene switch, because it negligibly prompts the innate immune system and doesn’t compromise the genome integrity.

The use of modRNA in primary and translational science is rising, because of the scientific potential of modRNA. We are presently utilizing modRNA to induce cardiac regeneration post-ischemic harm. Major obstacles in utilizing modRNA for cardiac ischemic illness embody the necessity for the direct and single administration of modRNA to the guts and the inefficient translation of modRNA attributable to its quick half-life.

Modulation of the 5′ untranslated area (5′ UTR) to boost translation effectivity in ischemic cardiac illness has nice worth, as it could scale back the quantity of modRNA wanted per supply and can obtain greater and longer protein manufacturing post-single supply.

Here, we recognized that 5′ UTR, from the fatty acid metabolism gene carboxylesterase 1D (Ces1d), enhanced the interpretation of firefly luciferase (Luc) modRNA by 2-fold in the guts post-myocardial infarction (MI).

Moreover, we recognized, in the Ces1d, a selected RNA component (component D) that’s accountable for the development of modRNA translation and results in a 2.5-fold translation increment over Luc modRNA carrying synthetic 5′ UTR, post-MI.

Importantly, we have been capable of present that 5′ UTR Ces1d additionally enhances modRNA translation in the liver, however not in the kidney, post-ischemic harm, indicating that Ces1d 5′ UTR and component D could play a wider position in translation of protein below an ischemic situation.

Optimization of 5' Untranslated Region of Modified mRNA for Use in Cardiac or Hepatic Ischemic Injury.
Optimization of 5′ Untranslated Region of Modified mRNA for Use in Cardiac or Hepatic Ischemic Injury.

Intradialytic neuromuscular electrical stimulation improves practical capability and muscle power in folks receiving haemodialysis: a scientific overview.

Does neuromuscular electrical stimulation (NMES) utilized throughout haemodialysis periods enhance practical capability in folks with end-stage renal illness? Does NMES used in this fashion additionally enhance muscle power, muscle mass/structure, psychological outcomes, cardiovascular outcomes and biochemical variables? Does it have any hostile results?Systematic overview of randomised managed trials with meta-analysis. PubMed, Web of Science, Scopus and SPORTDiscus have been searched from inception to 15 October 2019.Patients receiving haemodialysis for end-stage renal illness.

Horse IgG-Fc ELISA Kit, 96 tests, Quantitative
7715-Fc 1 kit
EUR 712

Dog IgG (Fc) fragment (isotype control, non-immune), purified
20016-FC 0.5 mg
EUR 250

Human IgG (Fc) fragment, purified
20007-1-FC 0.5 mg
EUR 208

Mouse IgG (Fc) fragment, purified
20008-1-FC 0.5 mg
EUR 250

Rabbit IgG (Fc) fragment, purified
20009-1-FC 0.5 mg
EUR 250

Goat IgG (Fc) fragment, purified
20011-1-FC 0.5 mg
EUR 250

Block
H5000-02 1 PC
EUR 290.33

Block
H5000-025 1 PC
EUR 591.35

Block
H5000-05 1 PC
EUR 290.33

Block
H5000-12 1 PC
EUR 382.55

Block
H5000-1232 1 PC
EUR 382.55

Block
H5000-15 1 PC
EUR 290.33

Block
H5000-150 1 PC
EUR 382.55

Block
H5000-20 1 PC
EUR 382.55

Block
H5000-500 1 PC
EUR 382.55

Block
H5000-CMB 1 PC
EUR 290.33

Block
H5000-CU 1 PC
EUR 382.55

Block
H5000-WB 1 PC
EUR 4288.85

Block
BSH100-02 1 PC
EUR 137.21

Block
BSH100-05 1 PC
EUR 146.78

Block
BSH100-1213 1 PC
EUR 137.21

Block
BSH100-1232 1 PC
EUR 137.21

Block
BSH100-15 1 PC
EUR 137.21

Block
BSH100-150 1 PC
EUR 156.35

Block
BSH100-1520 1 PC
EUR 137.21

Block
BSH100-15HL 1 PC
EUR 156.35

Block
BSH100-500 1 PC
EUR 104.15

Block
BSH100-5MT 1 PC
EUR 156.35

Block
BSH100-CV 1 PC
EUR 137.21

Block
BSW02 1 PC
EUR 151.93

Block
BSW05 1 PC
EUR 151.93

Block
BSW10 1 PC
EUR 151.93

Block
BSW13 1 PC
EUR 151.93

Block
BSW15 1 PC
EUR 151.93

Block
BSW1500 1 PC
EUR 151.93

Block
BSW1516 1 PC
EUR 183.03

Block
BSW1520 1 PC
EUR 151.93

Block
BSW50 1 PC
EUR 151.93

Block
BSW5MT 1 PC
EUR 151.93

Block
BSWHEM 1 PC
EUR 237.26

Block
BSWMT 1 PC
EUR 194.99

Block
BSWNMR 1 PC
EUR 481.73

Block
BSWPCR1 1 PC
EUR 255.6

Block
BSWPCR2 1 PC
EUR 208.55

Rat IgG (Fc) fragment, purified (isotype control)
20005-1-FC 0.5 mg
EUR 250

Goat IgG (fc)-Biotin conjugate (isotype control)
20011-Fc-B 100 ug
EUR 164

Goat IgG (fc)-FITC conjugate (isotype control)
20011-Fc-F 100 ug
EUR 164

Goat IgG (fc)-HRP conjugate (isotype control)
20011-Fc-HP 100 ug
EUR 164

Dog IgG (Fc) fragment (isotype control, non-immune), purified
20016-3-FC 0.5 mg
EUR 250

Dog IgG Fc-Biotin Conjugate (isotype control, non-immune), purified
20016-FC-B 0.1 mg
EUR 225

Dog IgG Fc-FITC Conjugate (isotype control, non-immune), purified
20016-FC-F 0.1 mg
EUR 225

Dog IgG Fc-HRP Conjugate (isotype control, non-immune), purified
20016-FC-HP 0.1 mg
EUR 225

EZ Block
EZB125 125 ml
EUR 93

EZ Block
EZB500 500 ml
EUR 127

EZ Block
EZB999 1000 ml
EUR 173

Pro-Block
PBK-20000 20 L
EUR 1210

Pro-Block
PBK010 10 L
EUR 676

Pro-Block
PBK125 125 ml
EUR 74

Pro-Block
PBK500 500 ml
EUR 101

Pro-Block
PBK999 1000 ml
EUR 124

Super Block
AAA125 125 ml
EUR 74

Super Block
AAA500 500 ml
EUR 101

Super Block
AAA999 1000 ml
EUR 124

Peroxide Block
ACA015 15 ml
EUR 67

Peroxide Block
ACA125 125 ea.
EUR 76

Peroxide Block
ACA500 500 ml
EUR 101

Peroxide Block
ACA999 1000 ml
EUR 125

Recombinant (HEK) Human IgG3-Fc (99-377aa, His-tag, >95%, low endotoxin)
20007-G3-FC 50 ug
EUR 286

Rat IgG (Fc)-Biotin Conjuagte (isotype control), purified
20005-1-FC-B 0.1 mg
EUR 225

Rat IgG (Fc)-FITC Conjuagte (isotype control), purified
20005-1-FC-F 0.1 mg
EUR 225

Rat IgG (Fc)-HRP Conjuagte (isotype control), purified
20005-1-FC-HP 0.1 mg
EUR 225

Human IgG (Fc) fragment, purified (>95%, low endotoxin)
20007-1-FC-LE 100 ul
EUR 225

Recombinant (HEK) Human IgG1 Fc (103Cys/Ser, 99-330aa, His-tag, >95%, low endotoxin)
20007-G1-FC 50 ug
EUR 286

Recombinant (HEK) Human IgG2-Fc (257Ser/Ala, 99-327aa, His-tag, >95%, low endotoxin)
20007-G2-FC 50 ug
EUR 286

Recombinant (HEK) Human IgG4-Fc (108Ser/Pro, 99-327aa, His-tag, >95%, low endotoxin)
20007-G4-FC 50 ug
EUR 286

Recombinant (HEK) Mouse IgG2a-Fc (98-330aa, ~34 kda, His-tag, >95%, low endotoxin)
20102-102-FC 50 ug
EUR 286

Recombinant (HEK) Mouse IgG2b-Fc (97-335aa, ~37 kda, His-tag, >95%, low endotoxin)
20102-103-FC 50 ug
EUR 286

Recombinant (HEK) Mouse IgG3-Fc (97-329aa, ~33 kda, His-tag, >95%, low endotoxin)
20102-104-FC 50 ug
EUR 286

Recombinant Human CD47 Protein, His-Fc (IgG), Insect-10ug
QP11336-HIS-Fc-10ug 10ug
EUR 201

Block, PCR plate, For 1-block dry bath
BCM1412-BSWPCR1 1 pcs, 1 UNIT
EUR 363.2

Block Wash Buffer
I044 1000 ml
EUR 527
Description: Block Wash Buffer by Cygnus Technologies is available in Europe via Gentaur.

HiQTM Immuno Block
I3211-010 100ml
EUR 134

HiQTM Immuno Block
I3211-050 500ml
EUR 266

Biotin Block Kit
B3810-002 30ml
EUR 219

Biotin Block Kit
B3810-005 120ml
EUR 408

Recombinant (HEK) Mouse IgG1-Fc (98-321aa, 102Cys/Ser, ~32 kda, His-tag, >95%, low endotoxin)
20102-101-FC 50
EUR 286

Human IgG Fc-Biotin conjugate (isotype control, non-immune), purified
20007-1-Fc-B 0.1 mg
EUR 164

Human IgG Fc-FITC conjugate (isotype control, non-immune), purified
20007-1-Fc-F 0.1 mg
EUR 164

Human IgG Fc-HRP conjugate (isotype control, non-immune), purified
20007-1-Fc-HP 0.1 mg
EUR 164

Human IgG (Fc) fragment-Biotin purified (>95%, low endotoxin)
20007-1-FC-LE-B 25 ug
EUR 286

Block, PCR plate, for 2 or 4-block dry bath
BCM1412-BSWPCR2 1 pcs, 1 UNIT
EUR 354.57

Block/Wash 20x Concentrate
F062 50 ml
EUR 195
Description: Block/Wash 20x Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Block/Wash 20x Concentrate
F062-100 100 ml
EUR 296
Description: Block/Wash 20x Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Block/Wash 20X Concentrate
F062-1000 1000 ml
EUR 411
Description: Block/Wash 20X Concentrate by Cygnus Technologies is available in Europe via Gentaur.

Cooling Block PCR WorkStation
LC4001 Ea
EUR 273

HiQ Block for IHC
B3077-010 100ml
EUR 134

HiQ Block for IHC
B3077-050 500ml
EUR 263

WSC-2610　MyMini BLOCK
4002610 1unit
EUR 655
Description: Compact si zed heat bl ock i ncubat or

Block-Free ELISA Reagent
M4102-250
EUR 452

Block-Free ELISA Reagent
M4102-50
EUR 147

Recombinant (HEK) Rabbit IgG (Fc) (101-323aa, 185Thr/Asn284Ser, ~33 kda, >95%, Low Endotoxin)
20009-1-R-FC 50 ug
EUR 286

Block, 15 x1.5ml centrifuge tubes
BCM1405-B15 1 pcs, 1 UNIT
EUR 162.3

Block, for 8 cuvettes (12.5x12.5x32mm)
BCM1405-BCV 1 pcs, 1 UNIT
EUR 162.3

Solid Block (for slides / machining)
BCM1412-BSW01 1 pcs, 1 UNIT
EUR 194.34

Peroxide Block for Image Analysis
ADA015 15 ml
EUR 71

Peroxide Block for Image Analysis
ADA500 500 ml
EUR 127

Peroxide Block for Image Analysis
ADA999 1000 ml
EUR 157

Block for 5ml centrifuge tubes
H5000-5MT 1 PC
EUR 382.55

Block for one Micro Plate
H5000-MP 1 PC
EUR 382.55

One-Block Digital Dry Bath
BSH1001-E 1 PC
EUR 520.88

Two-Block Digital Dry Bath
BSH1002-E 1 PC
EUR 831.47

Four-Block Digital Dry Bath
BSH1004-E 1 PC
EUR 151.93

Solid Block (for slides / machining)
BSW01 1 PC
EUR 182.23

Poly(lactide-co-glycolide)-block-poly(ethylene glycol)-block-poly(lactide-co-glycolide)
AV-2060-1 1 g
EUR 164

Plastic Columns Frits
FC-100 100/pk
EUR 122

Plastic Columns Frits
FC-50 50/pk
EUR 83

Fc
EF006811 96 Tests
EUR 689

Fc
EF006812 96 Tests
EUR 689

Fc
EF006814 96 Tests
EUR 689

Fc
EF006815 96 Tests
EUR 689

SINGLE BLOCK, 20 X 2.0ML TUBES
480117 1/pk
EUR 117
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 24 X 0.5ML TUBES
480118 1/pk
EUR 114
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 24 X 1.5ML TUBES
480119 1/pk
EUR 114
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 35 X 6MM TUBES
480120 1/pk
EUR 114
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 20 X 10MM TUBES
480121 1/pk
EUR 108
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 20 X 12MM TUBES
480122 1/pk
EUR 108
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 20 X 13MM TUBES
480123 1/pk
EUR 108
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 12 X 16MM TUBES
480125 1/pk
EUR 114
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 12 X 17MM TUBES
480126 1/pk
EUR 108
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 6 X 20MM TUBES
480127 1/pk
EUR 108
Description: Lab Equipment; Constant Temperature Equipment

SINGLE BLOCK, 6 X 25MM TUBES
480128 1/pk
EUR 108
Description: Lab Equipment; Constant Temperature Equipment

DUAL BLOCK, 384 WELL PCR PLATE
480133 1/pk
EUR 231
Description: Lab Equipment; Constant Temperature Equipment

Block, 24 x 0.5ml centrifuge tubes
BCM1405-B05 1 pcs, 1 UNIT
EUR 162.3

Block, 4 x 15ml centrifuge tubes
BCM1405-B150 1 pcs, 1 UNIT
EUR 189.41

Block, 2 x 50ml centrifuge tubes
BCM1405-B500 1 pcs, 1 UNIT
EUR 189.41

One-Block Digital Dry Bath 115V
BCM1412 1 pcs, 1 UNIT
EUR 616.42

Block, 24 x 0.5ml centrifuge tubes
BCM1412-BSW05 1 pcs, 1 UNIT
EUR 194.34

Block, 12 x 15ml centrifuge tubes
BCM1412-BSW15 1 pcs, 1 UNIT
EUR 194.34

Block, 24 x1.5ml centrifuge tubes (conical)
BCM1412-BSW1500 1 pcs, 1 UNIT
EUR 194.34

Block, 5 x 50ml centrifuge tubes
BCM1412-BSW50 1 pcs, 1 UNIT
EUR 194.34

Two-Block Digital Dry Bath 115V
BCM1413 1 pcs, 1 UNIT
EUR 745.83

Four-Block Digital Dry Bath 115V
BCM1414 1 pcs, 1 UNIT
EUR 1185.83

Immuno Diluent and Block (Clear), 50ml
NB307-C-50 50 ml
EUR 299

Peroxide Block (Stable) Ready-To-Use
HP1000-10 10ml
EUR 125

Peroxide Block (Stable) Ready-To-Use
HP1000-500 500 ml
EUR 485

Peroxide Block (Stable) Ready-To-Use
HP1000-60 60 ml
EUR 241

Evans Blue counter stain/diluent block
85-E09 3 ml
EUR 211
Description: Evans Blue counter stain/diluent block for use in immunofluorescent staining

CancerSeq? Paraffin Tissue Tumor Block: Breast
T2235086-SB 1 Block
EUR 4599
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

CancerSeq? Paraffin Tissue Tumor Block: Colon
T2235090-SB 1 Block
EUR 4599
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

CancerSeq? Paraffin Tissue Tumor Block: Lung
T2235152-SB 1 Block
EUR 4599
Description: Our tissue products are produced by strictly following the IRB ethical standards and procedures and from highest quality tissues. Immediately after collection the tissues are placed in liquid nitrogen and examined by certified pathologists. The thickness of each individual section is ~5um. They are Hematoxylin and Eosin stained and quality tested by immunostaining with anti-beta-actin antibodies. Our tissue products are suitable for various studies on cellular level (RNA localization, Protein expression, etc.) on both normal and pathological cases. It is also an excellent control and educational tool.

Solid Block. 1.25in/32mm in height
BSH100-01 1 PC
EUR 137.21

Block-Free ELISA Reagent (Protein-Free)
M4101-250
EUR 457

Block-Free ELISA Reagent (Protein-Free)
M4101-50
EUR 153

24x0.2ml Tube, accessory for DryBlock?
IPDB-Block-A
EUR 144

12x0.5ml Tube, accessory for DryBlock?
IPDB-Block-B
EUR 144

NMES administered throughout haemodialysis periods versus management.Functional capability, muscle power, muscle mass, psychological outcomes, cardiovascular outcomes, biochemical variables and hostile occasions.Data have been meta-analysed the place potential and outcomes have been expressed because the pooled imply distinction between teams with a 95% confidence interval.

Eight research (221 sufferers) have been included in the evaluation. Overall, the methodological high quality of the research was honest to good. NMES improved practical capability as assessed by the 6-minute stroll distance take a look at (MD 31 m, 95% CI 13 to 49) and peak workload attained in incremental train (MD 12.5 W, 95% CI 3.2 to 21.9). NMES elevated knee extensor muscle power (MD 3.5 kg, 95% CI 2.Three to 4.7) and handgrip power (MD 2.Four kg, 95% CI 0.Four to 4.4). Muscle mass/structure was not considerably affected.

NMES was estimated to be useful for a number of domains of high quality of life in a number of research, though most of these estimates have been imprecise. No advantages have been discovered for cardiovascular outcomes.

The accessible information didn’t set up any clear results on cardiovascular outcomes or biochemical variables (dialysis effectivity, urea and creatinine). No main NMES-related hostile occasions have been noticed.NMES is secure, sensible and efficient for enhancing practical capability and muscle power in haemodialysis sufferers.