Category: Uncategorized

Sources of Lab polyclonals

On: By: Brent
Extra-Large Aperture Assortment 10 each of 800 and 1000 um aperture Dual-Thickness MicroLoops
M5-L18SP-A6 MiTeGen 20 MOUNTS 112 EUR
Medium Aperture Assortment 5 each of 50, 100, 150 and 200 um aperture Dual-Thickness MicroLoops
M5-L18SP-A4 MiTeGen 20 MOUNTS 112 EUR
Sieve SS 200mm 13.2mm Aperture - EACH
SIE1002 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 11.2mm Aperture - EACH
SIE1004 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 9.5mm Aperture - EACH
SIE1006 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 8.0mm Aperture - EACH
SIE1008 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 6.7mm Aperture - EACH
SIE1010 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 5.6mm Aperture - EACH
SIE1012 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 4mm Aperture - EACH
SIE1016 Scientific Laboratory Supplies EACH 132.19 EUR
Sieve SS 200mm 2.8mm Aperture - EACH
SIE1020 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 2mm Aperture - EACH
SIE1024 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 1.7mm Aperture - EACH
SIE1026 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 1.4mm Aperture - EACH
SIE1028 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 1mm Aperture - EACH
SIE1032 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.6mm Aperture - EACH
SIE1038 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.5mm Aperture - EACH
SIE1040 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.3mm Aperture - EACH
SIE1046 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 16mm Aperture - EACH
SIE1000 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 4.75mm Aperture - EACH
SIE1014 Scientific Laboratory Supplies EACH 143.94 EUR
Sieve SS 200mm 3.35mm Aperture - EACH
SIE1018 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 2.36mm Aperture - EACH
SIE1022 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 1.18mm Aperture - EACH
SIE1030 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.85mm Aperture - EACH
SIE1034 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.71mm Aperture - EACH
SIE1036 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.425mm Aperture - EACH
SIE1042 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.355mm Aperture - EACH
SIE1044 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.25mm Aperture - EACH
SIE1048 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.212mm Aperture - EACH
SIE1050 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.15mm Aperture - EACH
SIE1054 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.106mm Aperture - EACH
SIE1058 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.09mm Aperture - EACH
SIE1060 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.075mm Aperture - EACH
SIE1062 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.063mm Aperture - EACH
SIE1064 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.045mm Aperture - EACH
SIE1068 Scientific Laboratory Supplies EACH 202.69 EUR
Sieve SS 200mm 0.038mm Aperture - EACH
SIE1070 Scientific Laboratory Supplies EACH 211.51 EUR
Sieve SS 200mm 0.10mm Aperture - EACH
SVMT511 Scientific Laboratory Supplies EACH 116.04 EUR
Sieve SS 200mm 0.20mm Aperture - EACH
SVMT519 Scientific Laboratory Supplies EACH 116.04 EUR
Screwcaps C/W Aperture Red - PK10
2922705 Scientific Laboratory Supplies PK10 25.65 EUR
MicroMount Small Aperture Assortment -5 each of 10, 20, 30 and 50 micron aperture mounts; mounted on 18 mm rods
M2-L18SP-A1 MiTeGen 20 MOUNTS 112 EUR
MicroMount Medium Aperture Assortment - 5 each of 75, 100, 150 and 200 micron aperture mounts; mounted on 18 mm rods
M2-L18SP-A2 MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; Small aperture assortment; 5 each of 20, 30, 50 and 75 micron aperture loops; mounted on 18 mm rods
M5-L18SP-A1LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; Medium aperture assortment; 5 each of 100, 150, 200 and 300 micron aperture loops; mounted on 18 mm rods
M5-L18SP-A2LD MiTeGen 20 MOUNTS 112 EUR
MicroLasso; 300 µm wide aperture. Box of 20.
T2-L25-12 MiTeGen 1 MicroTool 118 EUR
UV-VIS 100 Micron aperture MicroLoops (20 mounts total, on 18 mm pins)
MUV-L18SP-M5100 MiTeGen 20 MOUNTS 224 EUR
MicroGrippers; box of 20 with 100 micron aperture; mounted on 18 mm rods
M7-L18SP-100 MiTeGen 20 MOUNTS 112 EUR
MicroGrippers; box of 20 with 200 micron aperture; mounted on 18 mm rods
M7-L18SP-200 MiTeGen 20 MOUNTS 112 EUR
MicroGrippers; box of 20 with 300 micron aperture; mounted on 18 mm rods
M7-L18SP-300 MiTeGen 20 MOUNTS 112 EUR
MicroGrippers; box of 20 with 50 micron aperture; mounted on 18 mm rods
M7-L18SP-50 MiTeGen 20 MOUNTS 112 EUR
Lovibond TR 520 (8mm and 4mm Aperture) Reflectance Spectrocolorimeter - EACH
COL1022 Scientific Laboratory Supplies EACH 7981.46 EUR
Crystal Harvesting Sampler Kit 1 contains: 10 Dual-Thickness MicroMounts - 5 each of 50μm & 100μm apertures 5 MicroMeshes - 5 of the 400/25μm aperture 5 Dual-Thickness MicroLoops - 5 of the 200μm aperture 10 Dual-Thickness MicroLoops LD - 5 each of 50μm &
MSK-1 MiTeGen 1 KIT 214 EUR
MicroLoops LD; box of 20 with 100 micron aperture loops; mounted on 18 mm rods
M5-L18SP-100LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 150 micron aperture loops; mounted on 18 mm rods
M5-L18SP-150LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 200 micron aperture loops; mounted on 18 mm rods
M5-L18SP-200LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 20 micron aperture loops; mounted on 18 mm rods
M5-L18SP-20LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 300 micron aperture loops; mounted on 18 mm rods
M5-L18SP-300LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 35 micron aperture loops; mounted on 18 mm rods
M5-L18SP-35LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 50 micron aperture loops; mounted on 18 mm rods
M5-L18SP-50LD MiTeGen 20 MOUNTS 112 EUR
MicroLoops LD; box of 20 with 75 micron aperture loops; mounted on 18 mm rods
M5-L18SP-75LD MiTeGen 20 MOUNTS 112 EUR
Inclined MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods
M8-L18SP-15I MiTeGen 20 MOUNTS 112 EUR
Vertical MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods
M8-L18SP-15V MiTeGen 20 MOUNTS 112 EUR
Vertical MicroLoops E; box of 20 each 30x300 um aperture mounted on 18 mm rods
M8-L18SP-30V MiTeGen 20 MOUNTS 112 EUR
Inclined MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods
M8-L18SP-50I MiTeGen 20 MOUNTS 112 EUR
Vertical MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods
M8-L18SP-50V MiTeGen 20 MOUNTS 112 EUR
Vertical MicroLoops E; box of 20 each 70x700 um aperture mounted on 18 mm rods
M8-L18SP-70V MiTeGen 20 MOUNTS 112 EUR
Horizontal MicroLoops E; box of 20 each 15x150 um aperture mounted on 18 mm rods
M8-L18SP-15H MiTeGen 20 MOUNTS 112 EUR
Horizontal MicroLoops E; box of 20 each 50x500 um aperture mounted on 18 mm rods
M8-L18SP-50H MiTeGen 20 MOUNTS 112 EUR

Compare antibodies lab reagents for research

On: By: Brent



Suppliers for Lab ELISAs

HCV-IN-3
MBS3845392-1mg MyBiosource 1mg

HCV-IN-3
MBS3845392-5mg MyBiosource 5mg

HCV-IN-3
MBS3845392-5x10mg MyBiosource 5x10mg

HCV-IN-3
MBS5766792-5mg MyBiosource 5mg

HCV-IN-3
MBS5766792-5x5mg MyBiosource 5x5mg

HCV-IN-7
MBS5766799-5mg MyBiosource 5mg

HCV-IN-7
MBS5766799-5x5mg MyBiosource 5x5mg

Recombinant Humanp21 Recombinant Protein
92-035 ProSci 0.05 mg 821.4 EUR

TWEAK, recombinant / TNFSF12, recombinant (Human)
054-85 PHOENIX PEPTIDE 10 μg 261.36 EUR

TAGLN Recombinant Protein (Rat) (Recombinant- Tag)
RP232205 ABM 100 ug Ask for price

TAGLN2 Recombinant Protein (Rat) (Recombinant Tag)
RP232208 ABM 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)
RP232211 ABM 100 ug Ask for price

TAGLN3 Recombinant Protein (Rat) (Recombinant Tag)
RP232214 ABM 100 ug Ask for price

TAGAP Recombinant Protein (Human) (Recombinant- Tag)
RP030880 ABM 100 ug Ask for price

TAGLN Recombinant Protein (Human) (Recombinant- Tag)
RP030883 ABM 100 ug Ask for price

Our used monoclonals in Pubmed.

Anti- GPR162 Antibody Antibody
GWB-AAD3BE GenWay Biotech 0.05 ml Ask for price

Anti- GPR162 Antibody Antibody
GWB-C5010A GenWay Biotech 0.05 ml Ask for price

Anti-CD31 Antibody Antibody
MBS8304163-003mL MyBiosource 0.03mL 185 EUR

Anti-CD31 Antibody Antibody
MBS8304163-01mL MyBiosource 0.1mL 255 EUR

Anti-CD31 Antibody Antibody
MBS8304163-02mL MyBiosource 0.2mL 335 EUR

Compare Pcr lab reagents for research

On: By: Brent




Suppliers for Lab rec.

Lenti-EF1α-Sox2 Virus
G427 ABM 100 μl, Titer: 10^6 IU/ml
Description: N/A

Lenti-SFFV-KLF4 Virus
G432 ABM 100 μl, Titer: 10^6 IU/ml
Description: N/A

Lenti-SFFV-Oct4 Virus
G439 ABM 100 μl, Titer: 10^6 IU/ml
Description: N/A

Lenti-SFFV-Sox2 Virus
G440 ABM 100 μl, Titer: 10^6 IU/ml
Description: N/A

Lenti-III-EF1α Vector
LV043 ABM 10 μg

lenti MS2-P65-HSF1_Hygro
PVTY00190 Nova Lifetech 2ug

Lenti-EF1α-Nanog Virus
G424 ABM 100 μl, Titer: 10^6 IU/ml
Description: N/A

Monoclonal GR monoclonal antibody
AMM00029G Leading Biology 0.05mg 633.6 EUR

Monoclonal TBP monoclonal antibody
APR13720G Leading Biology 0.1ml 633.6 EUR

Monoclonal EZH2 monoclonal antibody
AMM00030G Leading Biology 0.05mg 633.6 EUR

Monoclonal Rsf1 monoclonal antibody
AMM07673G Leading Biology 0.05mg 633.6 EUR

Monoclonal Rsf1 monoclonal antibody
AMM07674G Leading Biology 0.1ml 633.6 EUR

Monoclonal HDAC2 monoclonal antibody
AMM00031G Leading Biology 0.05mg 633.6 EUR

Monoclonal SirT1 monoclonal antibody
APR09951G Leading Biology 0.05mg 580.8 EUR

Monoclonal SirT1 monoclonal antibody
APR09952G Leading Biology 0.1ml 580.8 EUR

Our used monoclonals in Pubmed.

Rec FLA-ST
tlrl-flic-10 InvivoGen FR 10 µg 295.05 EUR

Rec FLA-ST
tlrl-flic-50 InvivoGen FR 50 µg 731.85 EUR

rec EGF (human)
4030572.01 Bachem 0.1 mg 102.27 EUR

rec EGF (human)
4030572.05 Bachem 0.5 mg 271.85 EUR

rec EGF (human)
H-7490.0100 Bachem 0.1mg 194.4 EUR

Hologic Panther Fusion NATtrol

On: By: Brent

The expanding menu of Panther Fusion assays on the Panther Fusion system continues the legacy of testing precision and total lab automation that first began with the fully automated Panther system. Now, the flexibility of polymerase chain reaction (PCR) joins the power of transcription-mediated amplification (TMA) on the Panther Fusion system for multiple valuable chemistries on a single platform. These latest releases expand our diagnostic menu and consolidate testing to aid healthcare providers in diagnoses and maximize potential in the laboratory.

Leverage the benefits of the fully automated Panther Fusion system

The Panther Fusion system represents the latest innovation in Hologic diagnostic precision and automation. The Panther Fusion Respiratory assays leverage the benefits of the system as a whole, in addition to the assay-specific benefits.

Features include:

  • The combined flexibility of PCR and TMA
  • True sample-to-result automation
  • The ability to run only necessary assays and reduce expenses from unnecessary testing
  • Total nucleic acid extraction, with multiple tests from a single sample and multiplexing
  • Unit dose lyophilized reagents, which eliminate reagent waste and the need for manual reagent preparation
  • The ability to hold up to 28 cartridges, each with 12 reactions per cartridge for a total of 336 tests
  • Kits that can be reloaded as they are consumed without pausing testing

The Panther Fusion Respiratory assays

The Panther Fusion Respiratory assays for syndromic respiratory testing are the premier set of assays on the Panther Fusion system. You now can provide truly personalized patient testing with qualitative detection and differentiation of the most common respiratory viruses using a single patient sample. Each Panther Fusion Respiratory assay can be processed independently or simultaneously with other Panther Fusion and Aptima assays.

The Panther Fusion Respiratory menu leverages a legacy of expertise in respiratory viral molecular diagnostics from Hologic that began over a decade ago with the Prodesse Plus Series of respiratory assays.

Three respiratory assays comprise the IVD respiratory testing menu on the fully automated Panther Fusion system. Each is a multiplex, real-time  PCR in vitro diagnostic test. They isolate and purify nucleic acids from nasopharyngeal (NP) swab specimens obtained from patients who are symptomatic for respiratory tract infection. All 3 assays can be run from a single patient specimen loaded into the Panther Fusion system.

The Panther Fusion assays provide the flexibility to run patient-specific targets, allowing for personalized patient testing and better cost control in your lab.

  • The Panther Fusion Flu A/B/RSV assay – Qualitative detection and differentiation of influenza A virus, influenza B virus and respiratory syncytial virus
  • The Panther Fusion AdV/hMPV/RV assay – Qualitative detection and differentiation of adenovirus, human metapneumovirus and rhinovirus
  • The Panther Fusion Paraflu assay – Qualitative detection and differentiation of parainfluenza 1 virus, parainfluenza 2 virus, parainfluenza 3 virus, and parainfluenza 4 virus

Panther Fusion GBS assay

The Panther Fusion GBS assay joins the expanding molecular testing menu on the Panther Fusion system. This assay detects the gram-positive bacterium Streptococcus agalactiae (GBS) in pregnant women and so providers can prevent its transmission to their babies.4 GBS has serious long-term health consequences, including death, in newborns.

A Sensitive Assay for a Distinct Threat

This assay features world-class performance and sensitivity, answering a crucial need for GBS detection. GBS is a serious and sometimes fatal infection that is passed to infants during birth.5 GBS is a leading cause of early-onset neonatal sepsis in the United States, and it also can cause pneumonia and meningitis.5 Antibiotic prophylaxis can reduce transmission of GBS to babies when administered properly. The Panther Fusion GBS assay helps providers diagnose GBS in mothers so they can confidently determine if intrapartum prophylaxis is needed.

Intuitive Design for Real-World Need

The CDC states that accurate results are more important than rapid turnaround time for antenatal screening.5 When providers have diagnostic tools with excellent sensitivity and performance, they can deliver the highest level of care. The Panther Fusion GBS assay is a real-time PCR assay for antepartum testing with enriched vaginal and rectal swabs. It features dual-target detection of Cfb and SIP genes and 100% clinical sensitivity.4

True Flexibility and Full Automation

Run-on the Panther Fusion system, this assay offers the benefits of Panther Fusion automation, high-throughput flexibility, and ease of use, allowing for high-volume GBS testing in reference laboratories and hospitals. It may be processed, free from batching on the Panther Fusion system with other Aptima and Panther Fusion assays for women’s health, virology, respiratory, and sexually transmitted infections.

 

TYPES OF IMMUNITY

On: By: Brent

Immunoassays are a combination of biochemical techniques used to identify, and in other cases quantifiable, a certain molecular molecule present in a biological sample. The characteristic foundation which includes the types of immunity is that all of them are based on a specific anti-antigen reaction .

In this case, the 9 types of immunologicals that reveal more frequently in the laboratories of investigation should be reviewed.

TYPES OF IMMUNITY

1.- WESTERN BLOT (WB)

A Western Blot technician, the proteins presented in a determined biological sample are separated in the function of their median electrophoresis, to be transferred to a membrane and tissues with a specific anticancer.

With this technique, in order to detect the protein of interest, it is possible to determine its own, confirming whether there are post-translational modifications or comparing their concentration in different samples, between others.

2.- IMMUNOPRECIPITATION (IP)

Immunoprecipitation is used to determine the protein of the components present in a sample, taking into account the precipitation of anti-antigen compounds.

In addition to the detection and quantification of proteins, their applications are very different, including the determination of the molecular weight of a given antigen, the study of interactions between proteins or the monitoring of post-translational modifications.

3.- RADIOINMUNOENSAYO (RIA)

This immunoassay uses radioactive isotopes to mark the anti-antigen complex. It is important to follow the reading of radiation to detect these complexes and to confirm and / or quantify the presence of protein of interest in the sample.

One of the sales of Radioinmunoensayo is a very sensitive technique , which makes it possible to detect proteins that are found in other concentrations in the sample. Without embargo, it is also due to a practical importer, and it is a potential pilgrim that works with radioactive material.

4.- ELISA (ENZYME ENZYMES)

Of the different types of immunoassays, ELISA is one of the most popular and of the most frequently used research laboratories in their implementation, versatility and other sensitivities and specificities.

It is essential in the use of enzymes that allow the detection of anti-antigen unions (and therefore the presence of a certain antigen in the sample) to catalyze a colorimetric reaction to add the corresponding correspondence.

5.- CLIA (CHEMICAL IMMUNOSUPPRESSANTS)

The CLIA chemimuminescence tests, based on ELISA, differ in the case of this anti-antigen union measure, in addition to the generation of fluorescence generating a chemical reaction.

The CLIA examines the unconventional need for specific equipment to read the fluorescence, but in contrast to the sale of the significantly more sensible ELISAs.

6.- IMMUNOFLUORESCENCE (IF)

In immunofluorescence tests, the detection of the antigen of interest within the Union and of the specific anti-dumping shall be indicated by the use of fluorophores.

7.- IMMUNOHISTOCHEMISTRY (IHC)

These include a combination of immunological, biochemical and histological techniques for the detection of an antigen present in a section measuring the use of specific anti-dumping measures. Admittedly, it is possible to visualize the distribution and location of this antigen inside the studio.

8.- IMMUNOCYTOCHEMISTRY (ICC)

It is a technique with a foundation similar to the immunological chemistry, so that in this case, the immunocitochemistry allows the detection of an antigen inside a cell.

Thus, the IHC, as the ICC, is subject to less than any sensitivities to an ELISA or a Western blot, which may allow the antigen to be recorded in the context of a cell or one intact, not necessarily the need for an alarm.

9.- CITOMETRÍA DE FLUJO (FC)

Inside the different types of immunizations, it is one of the most complete. The flow cytometry is a method of dispersing and reflecting the light from the laser to analyze and analyze cells in suspension in the functioning of their physical-chemical characteristics.

 

 

TOP antibodies – AMACR (P504S) (clone 13H4) – rabbit monoclonal antibody

On: By: Brent

AMACR (P504S) (clone 13H4) – rabbit monoclonal antibody

Clone 13H4 – a classic that will not get bored         

 

AMACR (P504S) is an essential enzyme in the β-oxidation of branched chain fatty acids. High expression of the AMACR protein (P504S) has been demonstrated in prostate adenocarcinoma. Positivity is lacking in normal tissue, prostate tissue or benign lesions. AMACR (P504S) expression has also been demonstrated in two premalignant prostate lesions: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. The use of AMACR (P504S) as a positive marker along with basal cell staining (CK HMW or P63) as a negative marker may help to confirm the diagnosis of small-scale prostate cancer in a needle biopsy.

Positive control: kidney

Cellular localization: cytoplasmic

 

Human prostate cancer stained with anti-AMACR antibody using formalin-fixed peroxidase conjugate and DAB chromogen embedded in paraffin. Note the cytoplasmic and luminal peripheral staining of cancerous glands.